Lab protocols

Some frequently used protocols. For additional protocols, please email us.

Recommended resources

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Caenorhabditis Genetics Center (CGC)

C. elegans mutant consortium

The Caenorhabditis Genetics Center (CGC) is supported by the National Institutes of Health – Office of Research Infrastructure Programs (P40 OD010440). The CGC has been in operation since 1978, first at the University of Missouri, Columbia and since 1992 at the University of Minnesota, Twin Cities.

Ensembl genome browser

Ensembl is a genome browser for vertebrate genomes that supports research in comparative genomics, evolution, sequence variation and transcriptional regulation. Ensembl annotate genes, computes multiple alignments, predicts regulatory function and collects disease data. Ensembl tools include BLAST, BLAT, BioMart and the Variant Effect Predictor (VEP) for all supported species.

EpiGenie

Conferences and epigenetics news

EpiGenie is a team of folks who scour through PubMed, watch out for interesting epigenetics press releases, conduct interviews with epigenetics researchers, and work closely with the technology providers to stay on top of the most applicable work driving epigenetics-related research.

Expasy

Calculating molecular weight from amino acids

Million Mutation Project (MMP)

The Million Mutation Project (MMP), a joint project by the Moerman and Waterston labs, has exploited inexpensive whole genome resequencing to identify multiple mutations in virtually every C. elegans gene in a collection of 2,000 mutagenized strains.

National BioResource Project (NBRP)

The major aim of the National BioResource Project (NBRP) is to collect, preserve, and provide bio-resources (strains, populations, tissues, cells, genes of animals, plants and microorganisms, and information on these materials for R&D use) that are essential experimental materials for life science research.

NEXTDB

The Nematode Expression Pattern DataBase

NIH: BLAST

Compare proteins and DNA sequences

BLAST finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance.

PubMed

PubMed® comprises more than 34 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full text content from PubMed Central and publisher web sites.

TransgeneOme

The TransgeneOme is designed from the start as an open collaboration project that is intended to provide a platform for protein function discovery through crowd sourcing the task of strain generation, image acquisition and pattern annotation.

WormBase

C. elegans information

WormBase is an international consortium of biologists and computer scientists providing the research community with accurate, current, accessible information concerning the genetics, genomics and biology of C. elegans and related nematodes. Founded in 2000, the WormBase Consortium is led by Paul Sternberg (CalTech), Matt Berriman (The Wellcome Trust Sanger Institute), Sarah Dyer (EBI), and Lincoln Stein (The Ontario Institute for Cancer Research).

WormBook

WormBook is a comprehensive, open-access collection of original, peer-reviewed chapters covering topics related to the biology of Caenorhabditis elegans and other nematodes.